Journal: PLoS ONE
Article Title: IgG3 and IgM Identified as Key to SARS-CoV-2 Neutralization in Convalescent Plasma Pools
doi: 10.1371/journal.pone.0262162
Figure Lengend Snippet: (A) Neutralization titer of convalescent (n = 7) and pre-pandemic (n = 3) plasma pools. Data are shown as mean ± SD. (B) EUROIMMUN testing results (S1-IgG ELISA) for convalescent plasma pools (n = 7). (C) Ig classes of convalescent and pre-pandemic plasma pools against S1 ELISA (modified EUROIMMUN ELISA). Data are shown as mean ± SD. Asterisks indicate the level of statistical significance calculated using the Student’s t-test: *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001. (D) Representative plot for AUC calculation in a single pool sample showing transformed optical density values for different dilutions. AUC, area under the curve; Ig, immunoglobulin; LDL, lower detection limit; NT, neutralization titer; SD, standard deviation.
Article Snippet: ELISA data was analyzed using GraphPad Prism (8.1.1) and JMP software (15.1.0).
Techniques: Neutralization, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Modification, Transformation Assay, Standard Deviation